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The Elements of Bacteriological Technique
by John William Henry Eyre
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Bacteriological analyses, general considerations, 415 examination of blood, 377

Base of microscope, 50

Basidium, 128

Beer wort, preparation of, 175

Beetroot media, 200

Beggiotoa, morphology of, 133

Benzole bath, 256

Berkefeld filter, 42

Beyrinck's solution I, 197 II, 198

Bile salt agar (MacConkey), 205 broth, double strength, 199 (MacConkey), 180

Biochemical examination of cultures, 276

Biochemistry of bacteria, 276

Biological differentiation of bacteria, 249

Bipolar germination, 140

Bismarck brown, 94

Blastomycetes, morphology of, 129

Blood agar, 171, 214 plates, animal, 251 human, 250 (Washbourn), 214 bacteriological examination of, 377 cells, washing of, 388 collection of, for serological examination, 379 films, preparations of, 376 staining of, 97 histological examination of, 373 pipettes, 11 serological examination of, 378 stains, 97

Blood-serum (Councilman and Mallory), 208 inspissated, 168 (Loeffler), 208 (Lorrain Smith), 208

Blowpipe table, 9

Body tube of microscope, 50

Bohemian flask, 4

Boiling water, 33

Bone marrow, films, preparation of, 400

Bordet-Gengou reaction, 393

Boric acid in milk, test for, 442

Botkin's anaerobic method, 243

Bouillon, preparation of, 163

Brain extract, 149

Bread paste, 193

Brilliant green agar (Conradi), 206 bile salt agar (Fawcus), 206

Brownian movement, 79

Buchner's anaerobic method, 238

Bulloch's anaerobic method, 245 tubes for permanent preparations, 407

Bunge's mordant, 104

Burri's Chinese ink stain, 77

Butter, analysis of, 457 qualitative analysis of, 458 quantitative analysis of, 457

Cadaver, preparation of, for autopsy, 397

Cages for guinea-pigs, 343 for laboratory animals, 341 for mice, 342 for rabbits, 343 for rats, 342

Calculated figure for weight of medium mass, 166, 167

Cambier's candle method of isolating coli-typhoid groups, 438

Camera lucida, 62

Capaldi-Proskauer medium, No I, 186 No II, 187

Capillary pipettes, 10 graduated, 13

Capitate bacilli, 139

Capsule formation, 134 of bacteria, 134 thermo-regulator, 218

Capsules, collodion, inoculation of, 357 preparation of, 357 glass, 6 to clean infected, 20 new, 18 to stain, 99 to sterilise, 31

Carbohydrate media, preparation of, 177

Carbolic acid as a germicide, 27, 481 method of isolating coli-typhoid group, 437

Carbolised agar, 202 bouillon, 202 gelatine, 202

Carbon dioxide in cultures, test for, 289

Card index, 336, 402

Carrot media, 200

Cedarwood oil for immersion lens, 88

Cell wall of bacteria, 134

Celloidin sacs, manufacture of, 358

Cellular incubator, 216

Centrifugal machine for blood and serum work, 327 for milk work, 447

Centrifugalised milk, 449

Centrigade degrees, conversion of, 494

Chemical products of bacteria, 145

China green agar (Werbitski), 207

Chloroform as an antiseptic, 27

Chromatic aberration, 56

Chromogenic bacteria, 131

Chromoparous bacteria, 144

Chromophorous bacteria, 144

Citrated blood agar, 191

Cladothrix, morphology, 193

Classification of bacteria, 131 of fungi, 126

Clavate bacilli, 139

Clearing films with acetic acid, 82

Clostridium, 139

Coarse adjustment, 51

Cobweb micrometer, 66

Cocaine, 345

Cocci, morphology of, 131

Coccidium infection, 339

Coefficient, inferior lethal, 312 of inhibition, 311 phenol, 489 superior lethal, 313

Cohn's solution, 191

Cold incubator, 217

Coli-typhoid group, differential table, 433 in milk, 451 in soil, 477 isolation of, 432 members of, 430

Collection of blood for bacteriological examination, 378 for media making, 168 of milk samples, 443 of pathological material during life, 373 of pus, 373 of soil sample, 471 of water samples, 416

Collodion capsules, 357 sacs, manufacture of, 357

Colonies of bacteria, edges, 267

Coloured light, action of, 309

Columella, 127

Comparative haemocytology, 374

Complement, definition of, 325 fixation test, 393

Concentration method in water, analysis, 434

Condenser achromatic, 54 dark ground, 60 paraboloid, 60 substage, 54

Condidium, 128

Continuous sterilisation, 36

Contrast stains, 93

Corrosive sublimate (Lang), 82

Cotton-wool filter, 40

Counterstaining films, 84

Counting plate colonies, 423

Cover-slip films, 81 to clean new, 22 used, 24

Crates for test-tubes, 31

Cream, analysis of, 457 qualitative analysis of, 458 quantitative analysis of, 457

Crenothrix morphology, 133

Criteria of infection, 370

Criterion of immunity, 324

Cultural characters, macroscopical examination, 261

Culture flask, Guy's, 5 Kolle, 4 Roux, 5

Cuneate bacilli, 139

Cutaneous inoculation, 352

Dark ground condenser, 60 illumination, 87

Daughter cells, 129

Daylight, diffuse, action of, 308

Decimal scales, 340

Decolourising agents, 84

Definition of objective, 56

Depilatory powder, 346

Description of plate culture, 261

Descriptive terms, 261

Desiccation, effects of, 306 table, 501

Desiccator, Mueller's, 307

Dextrose solution, preparation of, 178

Diaphragm, iris, 53

Diastatic enzymes, tests for, 278

Differential atmosphere cultivation, 257 incubation, 255 media, 255 staining, 108 sterilisation, 256

Diluting chamber, 248

Dilution by teat pipette, 383 of serum, 382 tables, 498

Dilutions, preparations of, 496

Diphtheria, bacillus of, in milk, 452

Diplobacilli, morphology of, 133

Diplococci, morphology of, 133

Diplococcus pneumoniae, immunisation against, 322

Discontinuous sterilisation, 36

Discs of plaster-of-Paris, 192

Disinfectants, action of, 310 chemical, 27 testing of, 480

Dissociating fluid, Price Jones; 400

Dosage of inoculum, 316

Double nosepiece, 58 stains for spores, 106 sugar agar (Russell), 207

Drop-bottle, 73

Dry heat, 28

Dunham's solution, 177

Dyes, aniline, 83

Earthenware box for dirty slides, 70

Earthy salts agar (Lipman and Brown), 197

Edge of individual colonies, characters of, 267

Egg albumin agar, 213 broth, (Lipschuetz), 213 media (Dorset), preparation of, 174 inspissated, 212 (Lubenau), 209 (Tarchanoff and Kolesnikoff), 212 to clear nutrient media with, 166

Ehrlich's eyepiece, 55

Eikonometer, 65

Eisenberg's milk-rice medium, 189

Electric dental engine, 360 signal clock, 38 warm stage, 59

Elevation of colonies, 263

Eisner's gelatine, 204 method of isolating coli: typhoid group, 438

Endogenous spores, 138 varieties of, 139

Endo-germination, 139

English proof agar, Blaxall, 193

Enumerating colonies on plates, 423 discs, Jeffer's, 424 Pakes', 424

Enrichment method in water analysis, 427

Enumeration of micro-organisms, 423

Environmental conditions, 142

Enzyme production, investigation of, 277

Eosin, 93

Equatorial germination, 140

Erlenmeyer flask, 4

Ernstschen Koerner, 136

Esmarch's roll culture, 226 water collecting bottle, 417

Estimation of reaction of media, 280

Ether flame, 28 soluble acids, 284

Eucaine, 345

Exalting virulence of organisms, 320

Examination of milk, 441

Experimental infections, study of, during life, 370 inoculation of animals, 332

Extracellular toxins, 144

Eyepiece, Ehrlich, 55 micrometer, 63

Eyepieces, 55

Eye-shade, 57

Fahrenheit degrees, conversion of, 495

Feeding experiments, 369

Fermentation reactions, 279 tubes, 17

Field of objective, 56

Filar micrometer, 66

Filling tubes, etc., with medium, 160

Film preparations, 81 fixing, 81 making, 81 mounting, 85 staining, 83

Filter candle, closed, 47 open, 43 testing efficiency of, 478 to disinfect, 28 to sterilise, 29 flask, 6 papers, to fold, 156

Filters, cotton-wool, 40 porcelain, 42 testing of, 478

Filtration, 40 by aspiration, 42 of media, 156 under pressure, 45

Fine adjustment, 51 spindle head, 52

Fish, analysis of, 460 bouillon, 190

Fish gelatine, 190 gelatine-agar, 190

Fishing colonies, 253

Fission, reproduction by, 136

Fixation, 81 by heat, 81 of tissues, 114

Fixing fluids, for films, 82

Flagella, classification of bacilli by, 136 to stain, 101

Flask Bohemian, 4 Erlenmeyer, 4 filter, 6 Kitasato'a serum, 6 Kolle's culture, 4

Flasks and test tubes, to plug, 24 to clean dirty, 20 new, 18 to sterilise, 31

Fleischwasser, 148

Fluid cultures, description of, 271 media, 146

Foot of microscope, 50

Formaldehyde in milk, Hehner's test for, 442

Formalin method of preserving cultures, 407 tissues, 404

Fractional sterilisation, 33

Fraenkel and Voge's solution, 183

Fraenkel's earth borer, 472

Freezing method for sections, 115

French Mannite Agar (Sabouraud), 193 proof agar (Sabouraud), 193

Fresh preparations of bacteria, 74

Friedlaender's capsule stain for sections, 123

Frost's mounting fluid, 406

Frozen sections, rapid method, 116

Fuchsin, 92 agar (Braun), 205 sulphite agar (Endo), 206

Gas analysis, qualitative, 290 quantitative, 290 collecting apparatus, 291 generators, 242 production by bacteria, 289 tubes for media, 161

Gasperini's solution, 193

Gelatin agar, 193 preparation of, 164 surface plates, 231

General anaesthetics, 345

Gentian violet, 91

German lined paper, 69

Germicides, 27 testing power of, 480

Germination, 140

Geryk air-pump, 43

Glass apparatus in common use, 3 to clean, 18

Glass-cutting knife, 8

Glucose formate agar (Kitasato), 180 bouillon (Kitasato), 180 gelatine (Kitasato), 180

Glycerinated potato, 209

Glycerine agar, 209 blood-serum, 208 bouillon, 209 potato bouillon, 203 broth, 203

Goadby's gelatine, 214

Gonidium, 128

Goniodophore, 128

Graduated capillary pipettes, 13 pipettes, 6

Gram-Claudius' differential stain, 109

Gram's differential stain, 108

Gram-Weigert for sections, 121, 122

Gram-Weigert's differential stain, 109 modified, 110

Grease pencils, 72

Grouping of bacteria for study, 410

Guarded trepine, 360

Guarniari's agar gelatine, 194

Guinea-pig cages, 343 holder, 350

Gulland's solution, 82

Gum solution, preparation of, 116

Guy's culture bottle, 5

Gypsum blocks (Engel and Hansen), 192

Haematin, 95

Haematocytometer, 248

Haematoxilin, 95

Haemolysin, definition of, 326 preparation of, 327 storage of, 331

Haemolytic serum, titration of, 328

Hanging-block culture (Hill), 235

Hanging-drop cultures, 233 examination of, 86, 79 preparation of, 78 permanent staining of, 80 slides, 70

Hardening tissues, 114

Haricot agar, 200 bouillon, 200

Hay infusion, 200

Hearson's water bath, 299

Heat effect of, 299

Hehner's test, 442

Heiman's serum agar, 210

Hesse's anaerobic culture method, 237

Histological examination of blood, 373

Holder for guinea-pigs, 350

Hot air, 29 steriliser, 30 to use, 31 incubator, 217

Hot-water funnel, 158

Human blood agar plates, 250

Huyghenian eyepiece, 55

Hydrogen, generating apparatus, 242 in culture, test for, 289 peroxide in milk, test for, 442

Hyphomycetes, morphology of, 126 reproduction of, 126

Ice-box, for water samples, 419

Ice cream, analysis of, 457

Illuminant for microscope, 67

Immune body, 393

Immunisation, methods of, 321

Imperial system, 492 factors for converting, 493

Impression films, 85

Incubators, 216

Index cards, 336, 403

Indol, test for, 286

Infection, definition of, 370 general observations during life, 371 results of, 404

Influence of environment on bacterial growth, 142

Inhalation, fluid inoculum, 365 powdered inoculum, 366

Inhibition coefficient, 310, 311

Inoculation card index, 336 cutaneous, 352 intracranial, 360 intramuscular, 355 intraocular, 362 intraperitoneal, 355 intrapulmonary, 363 intravenous, 363 of collodion capsules, 357 subcutaneous, 353 syringe, 344

Inoculum, character of, 346 preparation of, 346

Inosite-free media—bouillon (Durham), 183

Inseparate toxins, 144

Intermittent sterilisation, 36

Intracellular toxins, 144

Intracerebral inoculation, 362

Intracranial inoculation, 360

Intragastric inoculation, large animals, 367 Marks method, 367

Intramuscular inoculation, 355

Intraocular inoculation, 362

Intraperitoneal inoculation, 355

Intrapulmonary inoculation, 363

Intravenous inoculation, 363

In vacuo anaerobia cultures, 289

Invertin enzymes, tests for, 279

Involution forms, 137

Iodine solution, 108

Iron bouillon, 185 peptone solution (Pakes), 185

Isolation by animal experiments, 258 by differential atmosphere, 257 incubation, 255 media, 255 sterilisation, 256 by dilution, 248 by plate cultures, 250 subcultures, preparation of, 254

Jeffer's counting disc, 424

Jenner's stain, 97

Jores' mounting fluid, 405

Kaiserling fixing solution, 405

Kanthack's serum agar, 211

Killed cultivations, 318

Kipp's hydrogen apparatus, 242

Kitasato's serum flask, 6

Klebs-Loeffler bacillus in milk, 452

Koch's steam steriliser, 34

Kohle's culture flask, 4

Lab enzymes, test for, 279

Laboratory animals, 335 comparative haematocytology of, 374 normal temperature, 372 regulations, 1

Lactose litmus agar (Wurtz), 203 bouillon, 203 gelatine (Wurtz), 203

Lakmus Molke, 203

Lang's solution, 82

Lead bouillon, 185 peptone solution, 186

Leishman's stain, 98 for sections, 125

Lemco broth, 163

Leptothrix, morphology, 133

Lethal dose, minimal, 316

Leviditi's staining method, 124

Light, action of, 308

Liquefiable media, 147

Liquid soap, 346

Lithium carmine, 96

Litmus bouillon, 186 gelatine, 202 milk cultures, description of, 272 preparation of, 172 nutrose agar (Drigalski-Conradi), 205 whey, 195 agar, 196 gelatine, 196 (Petruschky), 195

Local anaesthetics, 345 reaction to infection, 372

Locomotive movement, 80

Loeffler's capsule stain, 103 serum, 208

Lophotrichous bacilli, 136

Lorrain Smith electric warm stage, 59 serum, 208

Lugol's solution, to prepare, 108

Lysol, 27

MacConkey's capsule stain, 99 media, 180, 199, 205

MacCrorrie's capsule stain, 103

Macroscopical examination of cultures, 261

Malachite green agar (Loeffler), 207

Malt extract solution (Herschell), 196

Margin of individual colonies, 267

Martin's filtering apparatus, 320

Material for inoculation, 346

Mayer's albumin, 120

Mean phenol coefficient, 490

Measuring bacteria, 61

Meat, bacteriological analysis of, 460 extract preparation of, 148 reaction of, 149

Mechanical separation of bacteria, 249 stage, 52

Media, filtration of, 156 preparation of, 163 aerobic culture, 222 aesculin agar, 204 agar-agar, 167 agar gelatine (Guarniari), 194

Media, preparation of anaerobic culture, 180 animal tissue (Frugoni), 210 ascitic bouillon, 210 fluid agar (Wassermann), 213 asparagin (Fraenkel and Voge's), 183 (Uschinsky), 183 beer wort, 175 beetroot, 200 Beyrinck's solution I, 197 II, 198 bile salt agar (MacConkey), 205 broth (MacConkey), 180 double strength, 199 blood agar (Washbourn), 214 blood-serum, 168 (Councilman and Mallory), 208 (Loeffler), 208 (Lorrain Smith), 208 bouillon, 163 bread paste, 193 brilliant green agar (Conradi), 206 bile salt agar (Fawcus), 206 Capaldi-Proskauer, No. I, 186 No. II, 187 carbohydrate, 177 carbolised agar, 202 bouillon, 202 gelatine, 202 carrot, 200 China green agar (Werbitski), 207 citrated blood agar, 171 Cohn's solution, 191 dextrose solution, 178 double sugar agar (Russell), 207 earthy salt agar (Lipman and Brown), 197 egg Dorset, 174 Lubenau, 209 egg-albumen, inspissated, 212 (Tarchanoff and Kolesnikoff), 212 egg-albumin agar, 213 broth (Lipschuetz), 213 English proof agar (Blaxall), 193 fish bouillon, 190 gelatine, 190 agar, 190 fluid, 146 French mannite agar (Sabouraud), 193

Media, preparation of French proof agar (Sabouraud), 193 Fuchsin agar (Braun), 205 sulphite agar (Endo), 206 gelatine, 193 agar, 193 glucose formate agar (Kitasato), 180 bouillon (Kitasato), 180 gelatine (Kitasato), 180 glycerinated broth, 209 potato, 209 glycerine agar, 209 blood-serum, 208, 209 bouillon, 209 potato bouillon, 203 gypsum blocks (Engel and Hansen), 192 haricot agar, 200 bouillon, 200 hay infusion, 200 inosite free-bouillon (Durham), 183 iron bouillon, 185 peptone solution (Pakes), 185 lactose litmus agar (Wurtz), 203 bouillon, 203 gelatine (Wurtz), 203 lakmus molke, 203 lead bouillon, 185 peptone solution, 186 lemco broth, 163 liquefiable, 147 litmus bouillon, 186 gelatine, 202 milk, 172 nutrose agar (Drigalski-Conradi), 205 whey, 195 agar, 196 gelatine, 196 (Petruschky), 195 malachite green agar (Loeffler), 207 malt extract solution (Herschell), 196 milk, 172 rice (Eisenberg), 189 (Soyka), 189 Naegeli's solution, 191 Naehrstoff agar (Hesse and Niedner), 199 neutral litmus solution, 179 nitrate bouillon, 185 peptone solution (Pakes), 186 nutrient, 146 agar-agar, 167

Media, preparation of nutrient bouillon, 163 gelatine, 164 nutrose agar (Eyre), 172 oleic acid agar (Fleming), 201 Omeliansky's nutrient fluid, 189 Parietti's bouillon, 202 parsnip, 200 Pasteur's solution, 191 peptone rosolic acid water, 186 water (Dunham), 177 plaster-of-Paris discs, 192 potato, 174 gelatine (Elsner), 204 (Goadby), 214 proteid free broth (Uschinsky), 183 rosolic acid peptone solutions, 186 serum, bouillon, 210 dextrose water, (Hiss), 188 sugar, (Hiss), 188 water, 170 serum-agar (Heiman), 210 (Kanthack and Stevens), 211 (Libman), 212 (Wertheimer), 211 silicate jelly (Winogradsky), 198 solid, 147 special, 182 stock nutrient, 163 sugar, 177 agar, 185 (dextrose) bouillon, 184 gelatine, 184 sulphindigotate agar, 181 bouillon (Weyl), 181 gelatine (Weyl), 181 tissue (Noguchi), 214 turnip, 200 urine agar, 188 bouillon, 187 gelatine, 187 (Heller), 188 wheat bouillon (Gasperini), 193 whey agar, 195 gelatine, 195 wine must, 192 Winogradsky's solution (for nitric organisms), 198 (for nitrous organisms), 198 wood ash agar, 201 wort agar, 176 gelatine, 176

Media, preparation of yeast water (Pasteur), 191 standardisation of, 154 storage of, in bulk, 159 storing tubes of, 161 sore boxes, 162 titration of, 150 tubing of nutrient, 160

Merismopedia, morphology of, 132

Mesophilic bacteria, 143 pathogenic effects, 315

Metabolic end-products, 145

Metachromatic granules, 136

Metal instruments, to sterilise, 28

Metatrophic bacteria, 131

Methods of cultivation, 221 of identification of bacteria, 259 of inoculation, 352 of isolation, 248 of sterilisation, 26

Methylene-blue, 90

Metric system, 492 factors for converting, 493

Meyer's carmine, 96

Microbes of indication, 426

Micrococci, morphology, 132

Micrococcus, melitensis in milk, 456

Micrometer, filar, 66 net, 63 ocular, 63 stage, 62

Micrometry, methods of, 61

Micron, 61

Microscope, 49

Microscopical examination of bacteria, 86 stained, 88 unstained, 86 observations of cultures, 272

Milk, analysis of, qualitative, 446 quantitative, 444 condensed, analysis of, 444 media, 193 preparation of, 172 rice (Eisenberg), 193 (Soyka), 189 samples, collection of, 443 sedimenting tubes, 449

Minimal lethal dose, 316

Mirror for microscope, 55

Moeller's stain for spores, 107

Moist heat, 32

Molecular movement, 79

Monotrichous bacilli, 136

Motility, examination for, 79 true, 80

Moulds, examination of, 126 for paraffin imbedding, 117, 119

Mounting film preparations, 85 paraffin sections, 119

Mouse cages, 342 holder, 351 scales, 341

Mucor mucedo, 126

Mucorinae, 126

Mueller's desiccator, 307

Muffle furnace, 28

Muirs's capsule stain, 100 flagella stain, 101

Museum preparations of bacteria, 407 of tissues, 404 sealing of, 406

Mycelium, 126

Mycoprotein, 135

Naegeli's solution, 191

Naehrstoff agar (Hesse and Niedner), 199

Naked flame, 28

Neisser's stain modified, 111

Net micrometer, 63

Neutral litmus solution, preparation of, 179 red, 94

Nitrate bouillon, 185 peptone solution (Pakes), 186

Nitric organisms in soil, 478

Nitrosoindol reaction, 287

Nitrous organisms in soil, 477

Normal averages (t.p.r.), 372 serum, 375

Nosepiece, 57 double, 58 triple, 58

Navy's anaerobic method, 244 jars, 245

Nuclei, to stain, 105

Nucleus of bacteria, 135

Numerical aperture, 56

Nutrient media, 146

Nutrose agar (Eyre), preparation of, 172

Object marker, 61

Objectives, 55

Oblique tube cultures, 223

Ocular micrometer, 63

Oculars, 55

Oese, platinum, 71

Oidium, 128

Oil of garlic, 27 of mustard, 27

Oleic acid agar (Fleming), 201

Omeliansky's nutrient fluid, 189

Operation tables (Eyre's), 352 (Tatin's), 351

Opsonic index, 393

Opsonic index, determination of, 390

Opsonin, 387

Optical characters of colonies, 267

Optimum reaction of medium, determination of, 305 temperature, determination of, 298

Organisms of suppuration, 409

Orsat-Lunge gas apparatus, 292

Orth's carmine, 96

Oxford stain for Actinomyces, 112

Oysters, analysis of, 463

Pakes' counting disc, 424 filter reservoir, 45

Papier chardin, 158

Pappenheim's stain, 111

Paraboloid condenser, 60

Parachromophorous bacteria, 144

Paraffin method for sections, 117 sections, mounting of, 119 to stain, 121

Paratrophic bacteria, 131

Parietti's bouillon, 202 method of isolating coli-typhoid group, 437

Parsnip medium, 200

Passages of virus, 320

Pasteur-Chamberland filter, 42

Pasteur's pipettes, 10 solution, 191

Pathogenesis, investigation of, 315

Pathogenic bacteria, 131 study of, 408

Pediococci, morphology of, 132

Penicillium, 128

Peptone rosolic acid water, 186 water (Dunham), preparation of, 177

Percentage formula, 496

Perchloride of mercury, 27

Perisporaceae, 127

Peritrichous bacilli, 136

Permanent preparations of bacteria, 407 of tissues, 404

Petri's dishes, 6

Phagocytic index, 392

Phenol coefficient, 489 production, test for, 287

Photogenic bacteria, 131, 144

Physiological filter, 156

Picric acid solution, 121 (Spengler's), 112

Picrocarmine, 97

Pigment production, observations on, 288

Pipettes, automatic, 13 blood, 11 capillary, 10 cases for, 7 graduated, 6 capillary, 13 Pasteur's, 10 sedimentation, 16 standard graduated, 7 teat, 10 throttle, 13 to clean infected, 20 new, 18 to sterilise, 31

Piridin method of staining spirochaetes, 124

Pitfield's flagella stain, 103

Plasmolysis, 135

Plaster-of-Paris discs, 192

Plate box, 7 cultures, description of, 261 preparation of, 226 levelling stand, 228

Plates, Petri's, 6 to clean infected, 20 new, 18 to sterilise, 31

Platinum needles, 71 method of mounting, 71

Pleomorphism, 133

Polar germination, 140 granules, 136

Polkoerner, 136

Polychrome blood stains, 97

Pooled serum, 379

Porcelain filter, 42 Berkefeld, 42 Chamberland, 42 Doulton, 42

Post-mortem examination of experimental animals, 396

Potato gelatine (Eisner), 204 (Goadby), 214 medium, preparation of, 174

Potted meat, analysis of, 460

Pouring plates, 227

Preparation of experimental animals, 335

Preservatives in milk, 442

Pressure temperature table, 500

Primary colours, action of, 309

Proteid free broth (Uschinsky), 183

Proteolytic enzymes, tests for, 277

Prototrophic bacteria, 131

Psychrophilic bacteria, 143 pathogenic effects, 315

Pus, collection of, 373

Pyrogallic acid solution, 293

Qualitative analysis of air, 470 of milk, 446 of sewage, 467 of soil, 476 of unsound meat, 462 of water, 426

Quantitative analysis of air, 468 of milk, 444 of sewage, 466 of soil, 473 of unsound meat, 460

Rabbit cages, 343 scabies, treatment of, 338 scales, 340

Raising virulence of organisms, 320

Ramsden's micrometer, 66

Range of medium reaction, measurement of, 305 of temperature, measurement of, 298

Rat cages, 342

Raw milk, Saul's test for, 442

Reaction of medium, 305 optimum, 305 range of, 305 scale, 153

Reduced pressure and temperature table, 501

Reducing agents, production, 389 tests for, 289

Reduction of nitrates, 389

Reichert's thermo-regulator, 218

Relation of bacteria to environment, 142

Removal of material from culture tubes, 74

Rennin enzymes, tests for, 279

Reproduction of bacteria, 136

Resistance glass, 6 to lethal agents, 306

Resting stage of bacteria, 137

Restrictions upon experimental inoculations, 334

Ribbert's capsule stain, 101

Roll cultures, 226

Rosolic acid peptone solution, 186

Rosindol reaction, 286

Roux's anaerobic culture method, 237 culture bottle, 5

Sabouraud's medium, 193

Saccharomyces, morphology of, 129

Safranine, 94

Salicylic acid in milk, test for, 443

Saprogenic bacteria, 131

Sarcinae, morphology of, 132

Saul's test, 442

Scales, decimal, 340 trip, 164

Scalpels, to sterilise, 32, 33

Schallibaum's solution, 121

Scheme for study of bacteria, 259

Schizomycetes, classification of, 131 morphology of, 131

Scissors, to sterilise, 32

Sealing museum jars, 406

Searing iron, 397

Sections, special staining methods for, 121

Sedimentation pipettes, 16 tubes, 9

Selecting objectives, 57

Sensitising red blood cells, 395

Serial cultivations, 251

Serological examination of blood, 378

Serum agar (Heiman), 210 (Kanthack and Stevens), 211 (Libman), 212 plates, 250 (Wertheimer), 211 bouillon, 210 collection of, 379 dextrose water (Hiss), 188 inspissator, 169 sugar media (Hiss), 188 water, preparation of, 170

Sewage, analysis of, qualitative, 467 quantitative, 466

Shake cultivations, 225 description of, 271

Shape of colonies, 262

Shaving experimental animals, 349

Shellfish, analysis of, 463

Silicate jelly (Winogradsky), 198

Single stain for spores, 106

Size of colonies, 262

Slanted tube cultures, 223

Slides, to clean new, 22 used, 23

Smear culture, 224 description of, 268

Soap liquid, 346

Soda solution, storage of stock, 154

Sodium bicarbonate in milk, test for, 443

Soil, analysis of, qualitative, 476 quantitative, 473 collection of samples, 471

Solid media, 147

Soluble toxins, 144

Soyka's milk rice, 189

Spear-headed spatula, 402

Special media, 182

Specific serum, 379 dilution of, 382

Spherical aberration, 55

Spirillum, morphology of, 133

Spirochaeta, morphology of, 133

Spirochaetes in tissues, to stain, 124

Spleen extract, 149

Sporangium, 127

Spore formation, arthrogenous, 138 endogenous, 138 method of, 138, 273 germination, method of, 140, 274 observation of, 140, 273

Spores, characters of, 139 classification of, 139 double stain for, 106 to stain, 106

Stab culture, 224 description of, 265

Stage micrometer, 62 of microscope, 52

Staining methods, 90 paraffin sections, 121 reactions of bacteria, 274

Stains intra-vitam, 77 negative (Burri), 77 rack for, 72

Standard graduated pipettes, 7 soda solution, 154

Standardisation of media, 154

Standardising bouillon, 155

Staphylococci, morphology, 132

Staphylococcus in milk, 456

Steam steriliser, Arnold, 35 Koch, 35 to use, 35 streaming, 35

Sterigma, 127

Sterilisation by chemicals, 27 by dry heat, 28 by filters, 40 by moist heat, 32 by streaming steam, 35 by superheated steam, 36 of albuminous liquids, 32 of gases, 40

Sterilising agents, 26

Stichcultur, 224

Stock dilutions, 497 nutrient media, 163 plate for isolation work, 253

Storage of media in bulk, 159 of tubed media, 161

Store boxes for media, 161

Streak culture, 224 description of, 268

Streaming movement, 80 steam, 35

Streptobacilli, morphology, 133

Streptococci in soil, 477 in water, detection of, 432 morphology of, 132

Streptococcus pyogenes longus in milk, 455

Streptothrix, morphology of, 133

Strichcultur, 223

Structure, internal, of colonies, 265

Study of pathogenic bacteria, 408

Subcutaneous inoculation, 353

Subdural inoculation, 361

Substage condenser, 54

Sugar agar, 185 dextrose bouillon, 184 gelatine, 184 media, preparation of, 177

Sulphindigotate agar, 181 bouillon (Weyl), 181 gelatine (Weyl), 181

Sulphuretted hydrogen in cultures, test for, 290

Sunlight, action of, 309

Superheated steam, 36

Superior lethal coefficient, 310, 313

Suppuration, organisms of, 409

Surface characters of colonies, 264 plates, 230

Surgical motor, electric, 360

Swarm spores, 127

Syringe for subcutaneous inoculation of solid material, 354 hypodermic, 344

Tatin's operating table, 351

Taxonomy, 262

Teat-pipettes, 10

Temperature, action of, 299 optimum, 298 pressure table, 500 range, 298 taking, 340

Test objects for objectives, 57

Testing filters, 478

Test-tubes, 3 to clean infected, 19 new, 18 to plug, 24 to sterilise, 31

Tetracocci, morphology of, 132

Thermal death-point, 143 determination of, 298 of spores, 301, 304 of vegetative forms, 298, 303

Thermophilic bacteria, 143

Thermo-regulators, Hearson's capsule, 218 Reichert's, 218

Thionine blue, 92

Thiothrix, morphology of, 133

Thresh's water collecting bottle, 418

Throttle pipettes, 13

Tinned meat, analysis of, 460

Tissue medium (Noguchi), 214 stains, 95

Tissues for sectioning, fixing, 114 freezing, 116 hardening, 114 imbedding, 118 preparation of, 114 washing, 115

Titration of media, 150

Torulae, differentiation from saccharomyces, 130

Total acidity, 280

Toxins, testing of, 318

Trephines, 360

Triple nosepiece, 58

True motility, 80

Tube cultures, preparation of, 222 length, 50

Tubercle bacillus in milk, 453 to stain, 110, 124

Tuberculous guinea-pig, cadaver of, 454

Tubing nutrient media, 160

Turnip media, 200

Unna-Pappenheim's stain for sections, 123

Unsound meat, analysis of, 460

Urine agar, 188 gelatine, 187 (Heller), 188 media bouillon, 187

Uschinsky's solution, 183

Valency of specific sera, 386

Van Ermengem's flagella stain, 104

Vegetative stage of bacteria, 136

Vesuvin, 94

Vibrio cholerae in milk, 452 in water, 439 morphology of, 133

Virulence, attenuating, 321 of organisms, 320 raising, 320

Vivisection license, 334

Voges holder, 350

Volatile oils as disinfectants, 27

Warm stage, 58

Washing red blood cells, 388 tissues, 115

Water, analysis of, qualitative, 426 quantitative, 416 steriliser, 33

Weighing animals, 340

Welch's capsule stain, 101

Wertheimer's serum agar, 211

Wheat bouillon (Gasperini), 193

Whey agar, 195 gelatine, 195

Wine must, 192

Winogradsky's solution I, 198 II, 198

Wire crates for test-tubes, 31

Wood ash agar, 201

Working up plates, 252

Wort agar, 176 gelatine, 176

Wright's anaerobic method, 239

Yeast water (Pasteur), 191

Ziehl-Neelsen's stain, 110

Zoogloea, 134

Zymogenic bacteria, 131



SAUNDERS' BOOKS

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The excellent judgment displayed in the publications of the house at the very beginning of its career, and the success of the modern business methods employed by it, at once attracted the attention of leading men in the profession, and many of the most prominent writers of America offered their books for publication. Thus, there were produced in rapid succession a number of works that immediately placed the house in the front rank of Medical Publishers. One need only cite such instances as Musser and Kelly's Treatment, Keen's Surgery, Kelly and Noble's Gynecology and Abdominal Surgery, Cabot's Differential Diagnosis, De Lee's Obstetrics, Mumford's Surgery, Cotton's Dislocations and Joint Fractures, Crandon and Ehrenfried's Surgical After-treatment, Sisson's Veterinary Anatomy, Anders and Boston's Medical Diagnosis, Gant's Constipation and Obstruction, Jordan's Bacteriology, and Kemp on Stomach, Intestines, and Pancreas. These books have made for themselves places among the best works on their respective subjects.

A Complete Catalogue of our Publications will be Sent upon Request

Mallory's

Pathologic Histology

Pathologic Histology. By FRANK B. MALLORY, M. D., Associate Professor of Pathology, Harvard University Medical School. Octavo of 677 pages, with 497 figures containing 683 original illustrations, 124 in colors. Cloth, $5.50 net; Half Morocco, $7.00 net.

REPRINTED IN THREE MONTHS

Dr. Mallory here presents pathology from the morphologic point of view. He presents his subject biologically, first by ascertaining the cellular elements out of which the various lesions are built up; then he traces the development of the lesions from the simplest to the most complex. He so presents pathology that you are able to trace backward from any given end-result, such as sclerosis of an organ (cirrhosis of the liver, for example), through all the various acute lesions that may terminate in that particular end-result to the primal cause of the lesion. The illustrations are most beautiful.

Dr. W. G. MacCallum, Columbia University

"I have looked over the book and think the plan is admirably carried out and that the book supplies a need we have felt very much. I shall be very glad to recommend it."

* * * * *

Howell's Physiology

A Text-Book of Physiology. By WILLIAM H. HOWELL, PH.D., M. D., Professor of Physiology in the Johns Hopkins University, Baltimore, Md. Octavo of 1020 pages, 306 illustrations. Cloth, $4.00 net.

THE NEW (5th) EDITION

Dr. Howell has had many years of experience as a teacher of physiology in several of the leading medical schools, and is therefore exceedingly well fitted to write a text-book on this subject. Main emphasis has been laid upon those facts and views which will be directly helpful in the practical branches of medicine. At the same time, however, sufficient consideration has been given to the experimental side of the science. The entire literature of physiology has been thoroughly digested by Dr. Howell, and the important views and conclusions introduced into his work. Illustrations have been most freely used.

The Lancet, London

"This is one of the best recent text-books on physiology, and we warmly commend it to the attention of students who desire to obtain by reading a general, all-round, yet concise survey of the scope, facts, theories, and speculations that make up its subject matter."

Mallory and Wright's Pathologic Technique

Fifth Edition

Pathologic Technique. A Practical Manual for Workers in Pathologic Histology, including Directions for the Performance of Autopsies and for Clinical Diagnosis by Laboratory Methods. By FRANK B. MALLORY, M. D., Associate Professor of Pathology, Harvard University; and JAMES H. WRIGHT, M. D., Director of the Pathologic Laboratory, Massachusetts General Hospital. Octavo of 500 pages, with 152 illustrations. Cloth, $3.00 net.

In revising the book for the new edition the authors have kept in view the needs of the laboratory worker, whether student, practitioner, or pathologist, for a practical manual of histologic and bacteriologic methods in the study of pathologic material. Many parts have been rewritten, many new methods have been added, and the number of illustrations has been considerably increased.

Boston Medical and Surgical Journal

"This manual, since its first appearance, has been recognized as the standard guide in pathological technique, and has become well-nigh indispensable to the laboratory worker."

* * * * *

Eyre's Bacteriologic Technic

Bacteriologic Technic. A Laboratory Guide for the Medical, Dental, and Technical Student. By J. W. H. EYRE, M. D., F. R. S. Edin., Director of the Bacteriologic Department of Guy's Hospital, London. Octavo of 520 pages, 219 illustrations. Cloth, $3.00 net.

JUST READY—NEW (2d) EDITION, REWRITTEN

Dr. Eyre has subjected his work to a most searching revision. Indeed, so thorough was his revision that the entire book, enlarged by some 150 pages and 50 illustrations, had to be reset from cover to cover. He has included all the latest technic in every division of the subject. His thoroughness, his accuracy, his attention to detail make his work an important one. He gives clearly the technic for the bacteriologic examination of water, sewage, air, soil, milk and its products, meats, etc. And he gives you good technic—methods attested by his own large experience. To any one interested in this line of endeavor the new edition of Dr. Eyre's work is indispensable. The illustrations are as practical as the text.

McFarland's Pathology

A Text-Book of Pathology. By JOSEPH MCFARLAND, M. D., Professor of Pathology and Bacteriology in the Medico-Chirurgical College of Philadelphia. Octavo of 856 pages, with 437 illustrations, many in colors. Cloth, $5.00 net; Half Morocco, $6.50 net.

THE NEW (2d) EDITION

You cannot successfully treat disease unless you have a practical, clinical knowledge of the pathologic changes produced by disease. For this purpose Dr. McFarland's work is well fitted. It was written with just such an end in view—to furnish a ready means of acquiring a thorough training in the subject, a training such as would be of daily help in your practice. For this edition every page has been gone over most carefully, correcting, omitting the obsolete, and adding the new. Some sections have been entirely rewritten. You will find it a book well worth consulting, for it is the work of an authority.

St. Paul Medical Journal

"It is safe to say that there are few who are better qualified to give a resume of the modern views on this subject than McFarland. The subject-matter is thoroughly up to date."

Boston Medical and Surgical Journal

"It contains a great mass of well-classified facts. One of the best sections is that on the special pathology of the blood."

* * * * *

McFarland's Biology: Medical and General

Biology: Medical and General—By JOSEPH MCFARLAND, M. D., Professor of Pathology and Bacteriology in the Medico-Chirurgical College of Phila. 12mo, 457 pages, 160 illustrations. Cloth, $1.75 net.

JUST READY—NEW (2d) EDITION

This work is both a general and medical biology. The former because it discusses the peculiar nature and reactions of living substance generally; the latter because particular emphasis is laid on those subjects of special interest and value in the study and practice of medicine. The illustrations will be found of great assistance.

Frederic P. Gorham, A. M., Brown University.

"I am greatly pleased with it. Perhaps the highest praise which I can give the book is to say that it more nearly approaches the course I am now giving in general biology than any other work."

McFarland's Pathogenic Bacteria and Protozoa

Pathogenic Bacteria and Protozoa. By JOSEPH MCFARLAND, M. D., Professor of Pathology and Bacteriology in the Medico-Chirurgical College of Philadelphia. Octavo of 878 pages, finely illustrated. Cloth, $3.50 net.

NEW (7th) EDITION, ENLARGED

Dr. McFarland has subjected his book to a most vigorous revision, bringing this edition right down to the minute. Important new additions have increased it in size some 180 pages. By far the most important addition is the inclusion of an entirely new section on Pathogenic Protozoa. This section considers every protozoan pathogenic to man; and in that same clean-cut, definite way that won for McFarland's work a place in the very front of medical bacteriologies. The illustrations are the best the world affords, and are beautifully executed.

H. B. Anderson, M. D., Professor of Pathology and Bacteriology, Trinity Medical College, Toronto.

"The book is a satisfactory one, and I shall take pleasure in recommending it to the students of Trinity College."

The Lancet, London

"It is excellently adapted for the medical students and practitioners for whom it is avowedly written.... The descriptions given are accurate and readable."

* * * * *

Hill's Histology and Organography

A Manual of Histology and Organography. By CHARLES HILL, M. D., formerly Assistant Professor of Histology and Embryology, Northwestern University, Chicago. 12mo of 468 pages, 337 illustrations. Flexible leather, $2.00 net.

THE NEW (2d) EDITION

Dr. Hill's work is characterized by a completeness of discussion rarely met in a book of this size. Particular consideration is given the mouth and teeth.

Pennsylvania Medical Journal

"It is arranged in such a manner as to be easy of access and comprehension. To any contemplating the study of histology and organography we would commend this work."

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GET THE NEW THE BEST STANDARD American Illustrated Dictionary

New (7th) Edition—5000 Sold in Two Months

The American Illustrated Medical Dictionary. A new and complete dictionary of the terms used in Medicine, Surgery, Dentistry, Pharmacy, Chemistry, Veterinary Science, Nursing, and kindred branches; with over 100 new and elaborate tables and many handsome illustrations. By W. A. NEWMAN DORLAND, M.D., Editor of "The American Pocket Medical Dictionary." Large octavo, 1107 pages, bound in full flexible leather. Price, $4.50 net; with thumb index, $5.00 net.

IT DEFINES ALL THE NEW WORDS—IT IS UP TO DATE

The American Illustrated Medical Dictionary defines hundreds of the newest terms not defined in any other dictionary—bar none. These new terms are live, active words, taken right from modern medical literature.

It gives the capitalization and pronunciation of all words. It makes a feature of the derivation or etymology of the words. In some dictionaries the etymology occupies only a secondary place, in many cases no derivation being given at all. In the American Illustrated practically every word is given its derivation.

Every word has a separate paragraph, thus making it easy to find a word quickly.

The tables of arteries, muscles, nerves, veins, etc., are of the greatest help in assembling anatomic facts. In them are classified for quick study all the necessary information about the various structures.

Every word is given its definition—a definition that defines in the fewest possible words. In some dictionaries hundreds of words are not defined at all, referring the reader to some other source for the information he wants at once.

Howard A, Kelly, M. D., Johns Hopkins University, Baltimore.

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J. Collins Warren, M. D., LL.D., F.R.C.S. (Hon.), Harvard Medical School

"I regard it as a valuable aid to my medical literary work. It is very complete and of convenient size to handle comfortably. I use it in preference to any other."

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Stengel's Text-Book of Pathology

Fifth Edition

A Text-Book of Pathology. By ALFRED STENGEL, M. D., Professor of Medicine in the University of Pennsylvania. Octavo volume of 979 pages, with 400 text-illustrations, many in colors, and 7 full-page colored plates. Cloth, $5.00 net; Sheep or Half Morocco, $6.50 net.

WITH 400 TEXT-CUTS, MANY IN COLORS, AND 7 COLORED PLATES

In this work the practical application of pathologic facts to clinical medicine is considered more fully than is customary in works on pathology. While the subject of pathology is treated in the broadest way consistent with the size of the book, an effort has been made to present the subject from the point of view of the clinician. In the second part of the work the pathology of individual organs and tissues is treated systematically and quite fully under subheadings that clearly indicate the subject-matter to be found on each page. In this edition the section dealing with General Pathology has been most extensively revised, several of the important chapters having been practically rewritten.

The Lancet, London

"This volume is intended to present the subject of pathology in as practical a form as possible, and more especially from the point of view of the 'clinical pathologist.' These objects have been faithfully carried out, and a valuable text-book is the result. We can most favorably recommend it to our readers as a thoroughly practical work on clinical pathology."

* * * * *

Stiles' Nutritional Physiology

Nutritional Physiology. By PERCY GOLDTHWAIT STILES, Assistant Professor of Physiology at Simmons College, Boston. 12mo of 295 pages, illustrated. Cloth, $1.25 net.

ILLUSTRATED

This new work expresses the most advanced views on this important subject. It discusses in a concise way the processes of digestion and metabolism. The key-word of the book throughout is "energy"—its source and its conservation.

"It is remarkable for the fineness of its diction and for its clear presentation of the subject, relieved here and there by a quaintly humorous turn of phrase that is altogether delightful."—Colin C. Stewart, Ph. D., Dartmouth College.

* * * * *

Jordan's General Bacteriology

A Text-Book of General Bacteriology. By EDWIN O. JORDAN, PH.D., Professor of Bacteriology in the University of Chicago and in Rush Medical College. Octavo of 623 pages, illustrated. Cloth, $3.00 net.

NEW (3d) EDITION

Professor Jordan's work embraces the entire field of bacteriology, the non-pathogenic as well as the pathogenic bacteria being considered, giving greater emphasis, of course, to the latter. There are extensive chapters on methods of studying bacteria, including staining, biochemical tests, cultures, etc.; on the development and composition of bacteria; on enzymes and fermentation-products; on the bacterial production of pigment, acid and alkali; and on ptomaines and toxins. Especially complete is the presentation of the serum treatment of gonorrhea, diphtheria, dysentery, and tetanus. The relation of bovine to human tuberculosis and the ocular tuberculin reaction receive extensive consideration.

This work will also appeal to academic and scientific students. It contains chapters on the bacteriology of plants, milk and milk-products, air, agriculture, water, food preservatives, the processes of leather tanning, tobacco curing, and vinegar making; the relation of bacteriology to household administration and to sanitary engineering, etc.

Prof. Severance Burrage, Associate Professor of Sanitary Science, Purdue University.

"I am much impressed with the completeness and accuracy of the book. It certainly covers the ground more completely than any other American book that I have seen."

* * * * *

Buchanan's Veterinary Bacteriology

Veterinary Bacteriology. By ROBERT E. BUCHANAN, PH.D., Professor of Bacteriology in the Iowa State College of Agriculture and Mechanic Arts. Octavo, 516 pages, 214 illustrations. Cloth, $3.00 net.

THE BEST PUBLISHED

Professor Buchanan discusses thoroughly all bacteria causing diseases of the domestic animals. He goes minutely into the consideration of immunity, opsonic index, reproduction, sterilization, antiseptics, biochemic tests, culture-media, isolation of cultures, the manufacture of the various toxins, antitoxins, tuberculins, and vaccines that have proved of diagnostic or therapeutic value. Then, in addition to bacteria and protozoa proper, he considers molds, mildews, smuts, rusts, toadstools, puff-balls, and the other fungi pathogenic for animals.

B. F. Kaupp, D. V. S., State Agricultural College, Fort Collins.

"It is the best in print on the subject. What pleases me most is that it contains all the late results of research. It fills a long felt want."

Heisler's Embryology

A Text-Book of Embryology. By JOHN C. HEISLER, M.D., Professor of Anatomy in the Medico-Chirurgical College, Philadelphia. Octavo volume of 435 pages, with 212 illustrations, 32 of them in colors. Cloth, $3.00 net.

THIRD EDITION—WITH 212 ILLUSTRATIONS, 32 IN COLORS

This edition represents all the advances recently made in the science of embryology. Many portions have been entirely rewritten, and a great deal of new and important matter added. A number of new illustrations have also been introduced and these will prove very valuable. Heisler's Embryology has become a standard work.

G. Carl Huber, M.D., Professor of Embryology at the Wistar Institute, University of Pennsylania.

"I find this edition of 'A Text-Book of Embryology,' by Dr. Heisler, an improvement on the former one. The figures added increase greatly the value of the work. I am again recommending it to our students."

* * * * *

Boehm, Davidoff, and Huber's Histology

A Text-Book of Human Histology. Including Microscopic Technic. By DR. A. A. BOEHM and DR. M. VON DAVIDOFF, of Munich, and G. CARL HUBER, M.D., Professor of Embryology at the Wistar Institute, University of Pennsylvania. Handsome octavo of 528 pages, with 361 beautiful original illustrations. Flexible cloth, $3.50 net.

SECOND EDITION, ENLARGED

The work of Drs. Boehm and Davidoff is well known in the German edition, and has been considered one of the most practically useful books on the subject of Human Histology. This second edition has been in great part rewritten and very much enlarged by Dr. Huber, who has also added over one hundred original illustrations. Dr. Huber's extensive additions have rendered the work the most complete students' text-book on Histology in existence.

Boston Medical and Surgical Journal

"Is unquestionably a text-book of the first rank, having been carefully written by thorough masters of the subject, and in certain directions it is much superior to any other histological manual."

* * * * *

Wells' Chemical Pathology

Chemical Pathology.—Being a Discussion of General Pathology from the Standpoint of the Chemical Processes Involved. By H. GIDEON WELLS, PH.D., M.D., Assistant Professor of Pathology in the University of Chicago. Octavo of 616 pages. Cloth, $3.25 net.

JUST READY—NEW (2d) EDITION

Dr. Wells' work is written for the physician, for those engaged in research in pathology and physiologic chemistry, and for the medical student. In the introductory chapter are discussed the chemistry and physics of the animal cell, giving the essential facts of ionization, diffusion, osmotic pressure, etc., and the relation of these facts to cellular activities. Special chapters are devoted to Diabetes and to Uric-acid Metabolism and Gout.

Wm. H. Welch, M.D. Professor of Pathology, Johns Hopkins University.

"The work fills a real need in the English literature of a very important subject, and I shall be glad to recommend it to my students."

* * * * *

Lusk's Elements of Nutrition

Elements of the Science of Nutrition. By GRAHAM LUSK, PH.D., Professor of Physiology at Cornell Medical School. Octavo volume of 302 pages. Cloth, $3.00 net.

THE NEW (2d) EDITION—TRANSLATED INTO GERMAN

Prof. Lusk presents the scientific foundations upon which rests our knowledge of nutrition and metabolism, both in health and in disease. There are special chapters on the metabolism of diabetes and fever, and on purin metabolism. The work will also prove valuable to students of animal dietetics at agricultural stations.

Lewellys F. Barker, M. D. Professor of the Principles and Practice of Medicine, Johns Hopkins University.

"I shall recommend it highly to my students. It is a comfort to have such a discussion of the subject in English."

Daugherty's Economic Zoology

Economic Zoology. By L. S. DAUGHERTY, M. S., PH. D., Professor of Zoology, State Normal School, Kirksville, Mo., and M. C. DAUGHERTY, author with Jackson of "Agriculture Through the Laboratory and School Garden." Part I: Field and Laboratory Guide. 12mo of 237 pages, interleaved. Cloth, $1.25 net. Part II: Principles. 12mo of 406 pages, illustrated. Cloth, $2.00 net.

ILLUSTRATED

There is no other book just like this. Not only does it give the salient facts of structural zoology and the development of the various branches of animals, but also the natural history—the life and habits—thus showing the interrelations of structure, habit, and environment. In a word, it gives the principles of zoology and their actual application. The economic phase is emphasized.

Part I—the Field and Laboratory Guide—is designed for practical instruction in the field and laboratory. To enhance its value for this purpose blank pages are inserted for notes.

* * * * *

Drew's Invertebrate Zoology

A Laboratory Manual of Invertebrate Zoology. By GILMAN A. DREW, PH. D., Assistant Director at Marine Biological Laboratory, Woods Hole, Mass. With the aid of Former and Present Members of the Zoological Staff of Instructors. 12mo of 213 pages. Cloth, $1.25 net.

JUST READY—NEW (2d) EDITION

The subject is presented in a logical way, and the type method of study has been followed, as this method has been the prevailing one for many years.

Prof. Allison A. Smyth, Jr., Virginia Polytechnic Institute

"I think it is the best laboratory manual of zoology I have yet seen. The large number of forms dealt with makes the work applicable to almost any locality."

* * * * *

Norris' Cardiac Pathology

Studies in Cardiac Pathology. By GEORGE W. NORRIS, M.D., Associate in Medicine at the University of Pennsylvania. Large octavo of 235 pages, with 85 superb illustrations. Cloth, $5.00 net.

SUPERB ILLUSTRATIONS

The wide interest being manifested in heart lesions makes this book particularly opportune. The illustrations are superb and are faithful reproductions of the specimens photographed. Each illustration is accompanied by a detailed description; besides, there is ample letter press supplementing the pictures. Considerable matter of a diagnostic and therapeutic nature has been interwoven.

Boston Medical and Surgical Journal

"The illustrations are arranged in such a way as to illustrate all the common and many of the rare cardiac lesions, and the accompanying descriptive text constitutes a fairly continuous didactic treatise."

* * * * *

McConnell's Pathology

A Manual of Pathology. By GUTHRIE MCCONNELL, M.D., Professor of Bacteriology and Pathology at Temple University, Philadelphia. 12mo of 523 pages, with 170 illustrations. Flexible leather, $2.50 net.

NEW (2d) EDITION

Dr. McConnell has discussed his subject with a clearness and precision of style that make the work of great assistance to both student and practitioner. The illustrations have been introduced for their practical value.

New York State Journal of Medicine

"The book treats the subject of pathology with a thoroughness lacking in many works of greater pretension. The illustrations—many of them original—are profuse and of exceptional excellence."

* * * * *

Hektoen and Riesman's Pathology

AMERICAN TEXT-BOOK OF PATHOLOGY. Edited by LUDVIG HEKTOEN, M.D., Professor of Pathology, Rush Medical College, Chicago; and DAVID RIESMAN, M.D., Professor of Clinical Medicine, Philadelphia Polyclinic. Octavo of 1245 Pages, 443 illustrations, 66 in colors. Cloth, $7.50 net; Half Morocco, $9.00 net.

Duerck and Hektoen's Special Pathologic Histology

Atlas and Epitome of Special Pathologic Histology. By DR. H. DUERCK, of Munich. Edited, with additions, by LUDVIG HEKTOEN, M. D., Professor of Pathology, Rush Medical College, Chicago. In two parts. Part I.—Circulatory, Respiratory, and Gastro-intestinal Tracts. 120 colored figures on 62 plates, and 158 pages of text. Part II.—Liver, Urinary and Sexual Organs, Nervous System, Skin, Muscles, and Bones. 123 colored figures on 60 plates, and 192 pages of text. Per part: Cloth, $3.00 net. In Saunders' Hand-Atlas Series.

The great value of these plates is that they represent in the exact colors the effect of the stains, which is of such great importance for the differentiation of tissue. The text portion of the book is admirable, and, while brief, it is entirely satisfactory in that the leading facts are stated, and so stated that the reader feels he has grasped the subject extensively.

William H. Welch, M.D., Professor of Pathology, Johns Hopkins University, Baltimore.

"I consider Duerck's 'Atlas of Special Pathologic Histology,' edited by Hektoen, a very useful book for students and others. The plates are admirable."

* * * * *

Sobotta and Huber's Human Histology

Atlas and Epitome of Human Histology. By PRIVATDOCENT DR. J. SOBOTTA, of Wuerzburg. Edited, with additions, by G. CARL HUBER, M. D., Professor of Histology and Embryology in the University of Michigan, Ann Arbor. With 214 colored figures on 80 plates, 68 text-illustrations, and 248 pages of text. Cloth, $4.50 net. In Saunders' Hand-Atlas Series.

INCLUDING MICROSCOPIC ANATOMY

The work combines an abundance of well-chosen and most accurate illustrations, with a concise text, and in such a manner as to make it both atlas and text-book. The great majority of the illustrations were made from sections prepared from human tissues, and always from fresh and in every respect normal specimens. The colored lithographic plates have been produced with the aid of over thirty colors.

Boston Medical and Surgical Journal

"In color and proportion they are characterized by gratifying accuracy and lithographic beauty."

Bosanquet on Spirochaetes

Spirochaetes: A Review of Recent Work, with Some Original Observations. By W. CECIL BOSANQUET, M.D., Fellow of the Royal College of Physicians, London. Octavo of 152 pages, illustrated. $2.50 net.

ILLUSTRATED

This is a complete and authoritative monograph on the spirochaetes, giving morphology, pathogenesis, classification, staining, etc. Pseudospirochaetes are also considered, and the entire text well illustrated. The high standing of Dr. Bosanquet in this field of study makes this new work particularly valuable.

* * * * *

Levy and Klemperer's Clinical Bacteriology

The Elements of Clinical Bacteriology. By DRS. ERNST LEVY and FELIX KLEMPERER, of the University of Strasburg. Translated and edited by AUGUSTUS A. ESHNER, M. D., Professor of Clinical Medicine, Philadelphia Polyclinic. Octavo volume of 440 pages, fully illustrated. Cloth, $2.50 net.

S. Solis-Cohen, M.D., Professor of Clinical Medicine, Jefferson Medical College, Philadelphia.

"I consider it an excellent book. I have recommended it in speaking to my students."

* * * * *

Lehmann, Neumann, and Weaver's Bacteriology

Atlas and Epitome of Bacteriology: INCLUDING A TEXT-BOOK OF SPECIAL BACTERIOLOGIC DIAGNOSIS. By PROF. DR. K. B. LEHMANN and DR. R. O. NEUMANN, of Wuerzburg. From the Second Revised and Enlarged German Edition. Edited, with additions, by G. H. WEAVER, M. D., Assistant Professor of Pathology and Bacteriology, Rush Medical College, Chicago. In two parts. Part I.—632 colored figures on 69 lithographic plates. Part II.—511 pages of text, illustrated. Per part: Cloth, $2.50 net. In Saunders' Hand-Atlas Series.

* * * * *

Duerck and Hektoen's General Pathologic Histology

ATLAS AND EPITOME OF GENERAL PATHOLOGIC HISTOLOGY. By PR. DR. H. DUERCK, of Munich. Edited, with additions, by LUDVIG HEKTOEN, M. D., Professor of Pathology in Rush Medical College, Chicago. 172 colored figures on 77 lithographic plates, 36 text-cuts, many in colors, and 353 pages. Cloth, $5.00 net. In Saunders' Hand Atlas Series.

American Text-Book of Physiology Second Edition

AMERICAN TEXT-BOOK OF PHYSIOLOGY. In two volumes. Edited by WILLIAM H. HOWELL, PH. D., M.D., Professor of Physiology in the Johns Hopkins University, Baltimore, Md. Two royal octavos of about 600 pages each, illustrated. Per volume: Cloth, $3.00 net; Half Morocco, $4.25 net.

"The work will stand as a work of reference on physiology. To him who desires to know the status of modern physiology, who expects to obtain suggestions as to further physiologic inquiry, we know of none in English which so eminently meets such a demand."—The Medical News.

Warren's Pathology and Therapeutics Second Edition

SURGICAL PATHOLOGY AND THERAPEUTICS. By JOHN COLLINS WARREN, M. D., LL. D., F. R. C. S. (Hon.), Professor of Surgery, Harvard Medical School. Octavo, 873 pages, 136 relief and lithographic illustrations, 33 in colors. With an Appendix on Scientific Aids to Surgical Diagnosis and a series of articles on Regional Bacteriology. Cloth, $5.00 net; Half Morocco, $6.50 net.

Gorham's Bacteriology

A LABORATORY COURSE IN BACTERIOLOGY. For the Use of Medical, Agricultural, and Industrial Students. By FREDERIC P. GORHAM, A. M., Associate Professor of Biology in Brown University, Providence, R. I., etc. 12mo of 192 pages, with 97 illustrations. Cloth, $1.25 net.

"One of the best students' laboratory guides to the study of bacteriology on the market.... The technic is thoroughly modern and amply sufficient for all practical purposes."—American Journal of the Medical Sciences.

Raymond's Physiology New (3d) Edition

HUMAN PHYSIOLOGY. By JOSEPH H. RAYMOND, A. M., M. D., Professor of Physiology and Hygiene, Long Island College Hospital, New York. Octavo of 685 pages, with 444 illustrations. Cloth, $3.50 net.

"The book is well gotten up and well printed, and may be regarded as a trustworthy guide for the student and a useful work of reference for the general practitioner. The illustrations are numerous and are well executed."—The Lancet, London.

Ball's Bacteriology Seventh Edition, Revised

ESSENTIALS OF BACTERIOLOGY: being a concise and systematic introduction to the Study of Micro-organisms. By M. V. BALL, M. D., Late Bacteriologist to St. Agnes' Hospital, Philadelphia. 12mo of 289 pages, with 135 illustrations, some in colors. Cloth, $1.00 net. In Saunders' Question-Compend Series.

"The technic with regard to media, staining, mounting, and the like is culled from the latest authoritative works."—The Medical Times, New York.

Budgett's Physiology New (3d) Edition

ESSENTIALS OF PHYSIOLOGY. Prepared especially for Students of Medicine, and arranged with questions following each chapter. By SIDNEY P. BUDGETT, M. D., formerly Professor of Physiology, Washington University, St. Louis. Revised by HAVAN EMERSON, M. D., Demonstrator of Physiology, Columbia University. 12mo volume of 250 pages, illustrated. Cloth, $1.00 net. Saunders' Question-Compend Series.

"He has an excellent conception of his subject.... It is one of the most satisfactory books of this class"—University of Pennsylvania Medical Bulletin.

Leroy's Histology New (4th) Edition

ESSENTIALS OF HISTOLOGY. By LOUIS LEROY, M. D., Professor of Histology and Pathology, Vanderbilt University, Nashville, Tennessee. 12mo, 263 pages, with 92 original illustrations. Cloth, $1.00 net. In Saunders' Question-Compend Series.

"The work in its present form stands as a model of what a student's aid should be; and we unhesitatingly say that the practitioner as well would find a glance through the book of lasting benefit."—The Medical World, Philadelphia.

Barton and Wells' Medical Thesaurus

A THESAURUS OF MEDICAL WORDS AND PHRASES. By WILFRED M. BARTON, M. D., Assistant Professor of Materia Medica and Therapeutics, and WALTER A. WELLS, M. D., Demonstrator of Laryngology, Georgetown University, Washington, D.C. 12mo, 534 pages. Flexible leather, $2.50 net; thumb indexed, $3.00 net.

American Pocket Dictionary New (8th) Edition

DORLAND'S POCKET MEDICAL DICTIONARY. Edited by W. A. NEWMAN DORLAND, M. D., Editor "American Illustrated Medical Dictionary." Containing the pronunciation and definition of the principal words used in medicine and kindred sciences, with 64 extensive tables. 677 pages. Flexible leather, with gold edges, $1.00 net; with patent thumb index, $1.25 net.

"I can recommend it to our students without reserve."—J. H. HOLLAND, M.D., of the Jefferson Medical College, Philadelphia.

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